study on some immunological responses of rainbow trout (onchorhynchus mykiss) to some antigens of streptococcus iniae
نویسندگان
چکیده
some specific and non-specific immune variables of rainbow trout were assessed following vaccination of fish with formalin killed cells (fkc) and fkc containing extra cellular products (ecp) of s. iniae. rainbow trout weighing 80-100 g were vaccinated by intraperitoneal (i.p), dip and oral routes using fkc and fkc plus ecp with or without fround adjuvant (fa) at 16-17°c.antibody titration, lysozyme activity, serum bacterial killing activity and population of immunocompetent cells were measured on 3, 6, 9, 12, 15 and 18 weeks postvaccination. data were analyzed by anova. results showed that the highest antibody titers were produced in i.p vaccinated fish with fkc plus ecp and immunized fish with fkc by dip, respectively. however, oral administration resulted in the lowest response. also, the level of antibody production was higher during initial period of post-immunization, while it reduced to lower levels at the end of sampling time. similar results were obtained when lysozyme activity and bacterial killing capacity of sera samples were estimated. moreover, while,leukocyte and lymphocyte populations in immunized fish were generally higher than control groups, heterophil and monocyte counts were varied during the sampling periods.reuslts show that both humeral and cellular immunities of trout are enhanced following immunization of fish with fkc with or without ecp administered as i.p and dip.however, i.p administration of fkc with or without ecp could cause higher response than both dip and oral routes.
منابع مشابه
Genetic Characterization of Streptococcus iniae in Diseased Farmed Rainbow Trout (Onchorhynchus mykiss) in Iran
Genetic characterization of strains of Streptococcus iniae recovered from morbidity and mortality of farmed rainbow trout in different provinces of Iran were studied. The Gram-positive cocci isolates were obtained from the kidney tissues of diseased rainbow trout on blood agar at 25°C for 72 h. The grown bacteria were then characterized using biochemical and molecular works. The identified 26 i...
متن کاملSurvey on viability of Streptococcus iniae in frozen rainbow trout (Oncorhynchus mykiss) following experimental infection
Streptococcus iniae is one of the most important agents that causes high mortality and losses in rainbow trout farms in Iran and the world. These bacteria can affects the consumers and people who deal with aquaculture via affected fish. So, viability of this bacterium in frozen fish and fish product can be useful for health care of consumers. A total of 90 rainbow trout, with average weight of ...
متن کاملHumoral and non-specific immune responses in rainbow trout (Oncorhynchus mykiss) naturally exposed to and immunized with Streptococcus iniae
In this study, immune responses against Streptococcus iniae infections in rainbow trout (Oncorhynchus mykiss) were analyzed. Anti-streptococcal antibody (ASA) levels, respiratory burst activity (RBA), phagocytic activity (PA) and phagocytic index (P index) were measured in kidney macrophages. Fish were allocated to three experimental groups (n = 50 per group): survivors of natural S. iniae inf...
متن کاملSerum antibody response of rainbow trout, Oncorhynchus mykiss to two species of pathogenic bacteria; Streptococcus iniae and Lactococcus garviae
One of the most important bacterial diseases in aquaculture is Streptococosis/loctococosis. Meanwhile this disease has been known as a zoonosis disease, and then fish vaccination against this disease is important. In this study the specific immunological responses of rainbow trout (Oncorhynchus mykiss) to vaccination with bivalent lactococcal/streptococcal vaccine were investigated. Two hundred...
متن کاملLactococcus garvieae and Streptococcus iniae infections in rainbow trout Oncorhynchus mykiss: similar, but different diseases.
Clinical and macroscopic findings (anorexia, lethargy, loss of orientation and exophthalmia) indicate that Streptococcus iniae and Lactococcus garvieae infections of trout share some common features, but histopathology reveals notable differences between the 2 diseases. Meningitis and panophthalmitis are the main lesions among S. iniae infected trout, whereas L. garvieae infection results in a ...
متن کاملextensions of some polynomial inequalities to the polar derivative
توسیع تعدادی از نامساوی های چند جمله ای در مشتق قطبی
15 صفحه اولمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
تحقیقات دامپزشکیجلد ۶۲، شماره ۱، صفحات ۱-۹
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023